Molecular Cloning and Expression Analysis of an Actin Gene from Chinese Licorice（Glycyrrhiza uralensis Fisch）

A PCR-based homologous cloning strategy was used to identify an actin gene from the roots of Chinese licorice(Glycyrrhiza uralensis Fisch). Results of sequence analysis indicate that a 1137 bp cDNA with an open reading frame encoding 377 amino acids,actin ortholog,GuActin,was successfully cloned and characterized(GenBank accession No. EU190972). Thus far,GuActin is the first actin of Chinese licorice that has been identified at a molecular level. Analysis by Northern blot shows that GuActin was expressed st...     

CAUCHY PROBLEM FOR LINEARIZED SYSTEM OF TWO-DIMENSIONAL ISENTROPIC FLOW WITH AXISYMMETRICAL INITIAL DATA IN GAS DYNAMICS

§1IntroductionIn the present paper,we are interested in solving the Cauchy problem for linearizedsystem of two-dimensional isentropic flow with initial data in gas dynamicsρt+ρ0xu+yv=0,ut+p′(ρρ00)xρ=0,vt+p′(ρ0ρ0)yρ=0,(1.1)t=0:(ρ,u,v)=(ρ0(r),u0(r),v0(r)),(1.2)whereρis the density,(u,v)is the velocity,ρ0is a positive constant,p=p(ρ)is theequation of state satisfying p′(ρ0)>0,(r,θ)is the polar coordinate such thatx=rcosθ,y=rsinθ,0≤r<+∞,0≤θ≤2π…     

Thermodynamically conditioned splitting schemes in combustion problems

Algorithms for solving the two-dimensional combustion problem for premixed flames are proposed and examined. The solution method is based on splitting into convective and diffusion parts according to the processes involved. A high-resolution explicit quasi-monotone scheme with flux correction is used for the hyperbolic part. For the parabolic part, the scheme is conservative and the source in the heat equation is set to be positive; i.e., the scheme ensures that the different thermodynamic consequences of the original equations hold; therefore, the scheme is thermodynamically conditioned. The applicability of the scheme to the full and purely gasdynamic problems is examined under various types of initial conditions and with various flux limiters. Numerical results are presented for one-and two-dimensional problems, including the Frank-Kamenetskii classical problem in two dimensions. The flame is shown to become turbulent in sufficiently wide pipes.     

Expression analyses of human cleft palate tissue suggest a role for osteopontin and immune related factors in palatal development

Cleft lip and/or palate (CL/P) is a common congenital malformation with a complex etiology which is not fully elucidated yet. Epidemiological studies point to different etiologies in the cleft lip and palate subgroups, isolated cleft lip (CL), isolated cleft palate (CP) and combined cleft lip and palate (CLP). In order to understand the biological basis in these cleft lip and palate subgroups better we studied the expression profiles in human tissue from patients with CL/P. In each of the CL/P subgroups, samples were obtained from three patients and gene expression analysis was performed. Moreover, selected differentially expressed genes were analyzed by quantitative RT-PCR, and by immunohistochemical staining of craniofacial tissue from human embryos. Osteopontin (SPP1) and other immune related genes were significantly higher expressed in palate tissue from patients with CLP compared to CP and immunostaining in palatal shelves against SPP1, chemokine receptor 4 (CXCR4) and serglycin (PRG1) in human embryonic craniofacial tissue were positive, supporting a role for these genes in palatal development. However, gene expression profiles are subject to variations during growth and therefore we recommend that future gene expression in CL/P studies should use tissue from the correct embryonic time and place if possible, to overcome the biases in the presented study.     

碱基序列标记法结合焦磷酸测序测定不同来源基因表达量

建立了一种将序列标记反转录聚合酶链反应(PCR)与焦磷酸测序技术结合的相对基因表达量测定法(简称"SRPP").先用来源特异性引物对不同来源的同一基因通过反转录标记上特异性标签,PCR后用焦磷酸测序法对扩增产物进行序列解码,使得测序结果中的序列代表基因的来源,峰高代表基因在不同来源中的相对表达量.用实时荧光定量PCR法对本方法的准确性进行了验证,结果表明,SRPP可以同时准确测定同一基因在3个不同来源中的表达量,并实际测定了Egr1基因在糖尿病、肥胖和正常小鼠肝中的表达量差异.     

Conservation laws, hierarchy of dynamics and explicit integration of nonholonomic systems

This paper can be regarded as a continuation of our previous work [1, 2] on the hierarchy of the dynamical behavior of nonholonomic systems. We consider different mechanical systems with nonholonomic constraints; in particular, we examine the existence of tensor invariants (laws of conservation) and their connection with the behavior of a system. Considerable attention is given to the possibility of conformally Hamiltonian representation of the equations of motion, which is mainly used for the integration of the considered systems.      

热传导方程的小波解法

本文利用微分算子的小波表示，讨论一维热传导方程初值问题的Daubechies小波解，给出此问题的显式离散格式。由于小波在时间和频率上的局部性，此方法特别适用于有奇异解的热传导方程，逼近精度高，而且没有发生解的振荡现象。     

Immune Responses to a Dicistronic Plasmid Expressing HBsAg of Hepatitis B Virus and Interferon-γ

DNA vaccines encoding a viral protein have been shown to induce antiviral immune responses and provide protection against subsequent viral challenge.The present article deals with the efficacy of a DNA vaccine greatly improved by the simultaneous expression of HBsAg and interferon-γ gene.We constructed a dual expression vector pHIN encoding the HBsAg of Hepatitis B virus and murine IFN-γ which are connected with Internal Ribosome Entry Site(IRES).Mice immunized with this dual expression DNA vaccine exhibited the enhancement of cellular immune response and increased the production of anti-HBV surface antibody,compared with the mice of single gene expression control.Taken together,these results demonstrate that the application of a cytokine gene in a DNA vaccine formulation as an adjuvant can improve its immunigenicity.